Coppercube position x percent
This finding might underscore metal dysregulation, beyond iron, in cells derived from FRDA patients. Interestingly, no significant difference in the mean iron concentration was found in the cytoplasm of FRDA fibroblasts, but a significant decrease in zinc concentration. Despite the presence of diffracting microcrystals in the vitreous ice matrix and minor sample radiation damage effects, clusters of iron-rich hot-spots with similar mass fractions were found in the cytoplasm of both control and FRDA fibroblasts. By means of the X-ray Fundamental Parameter (FP) method, including absorption correction of the ice layer deposited onto the fibroblasts, background-corrected mass fraction elemental maps of P, S, Cl, K, Ca, Fe and Zn of entire cryofrozen human fibroblasts were obtained. FRDA is an autosomal recessive disorder with dysregulation of iron metabolism as a key feature.
#Coppercube position x percent skin#
Fibroblast cells were obtained from skin biopsies from control and Friedreich’s ataxia (FRDA) patients. Here, we describe first nanoscopic XRF analysis upon cryogenically frozen (-150☌) fibroblasts at the ID16A-NI ‘Nano-imaging’ end-station located at the European Synchrotron Radiation Facility (ESRF) in Grenoble (France). However, in-air XRF analysis requires chemical fixation modifying the cell’s chemical composition.
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Synchrotron radiation based nanoscopic X-ray fluorescence (SR nano-XRF) analysis can visualize trace level elemental distribution in a fully quantitative manner within single cells.